Useful For
In vitro test for the quantitative determination of calcium in human serum, plasma and urine on Roche/Hitachi cobas c systems.
Method Name
Calcium ions react with 5-nitro-5’-methyl-BAPTA (NM-BAPTA) under alkaline conditions to form a complex. This complex reacts in the second step with EDTA. The change in absorbance is directly proportional to the calcium concentration and is measured photometrically.
Aliases
Calcium, Urine 24 Hour, Calcium - 24hr Urine
Specimen Type
Urine
Specimen Required
urine in a 24 hour urine container
Specimen Minimum Volume
Submit entire collection for a 24 hour period
Specimen Stability Information
Stable at room temperature for 2 days and for 4 days refrigerated.
Special Instructions
1. To start the collection, empty bladder and discard this specimen. This will be the start of the collection period. Write the date and time on the collection container. 2. DO NOT VOID directly into the container. Void into a clean container and then pour the specimen into the 24 container. Keep the container refrigerated during the collection period. 3. At precisely the same time that the collection was started 24 hours earlier, empty bladder one final time and add this specimen to the 24 hour collection container. 4. Deliver container to the Laboratory.
Performing Laboratory
Chemistry
Reference Values
100-300 mg/24 hr
Day(s) and Time(s) Performed
As ordered, 4 hour turn around time.
Specimen Retention Time
72 hours